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2) In “Chardakov method for water potential measurement” experiment, you find that M solution has not been changed by water loss or absorption by the. Chardakov Technique. Plant Physiology. UNI. Incubating tissue in solution. Pre- incubation. Incubation. Post-incubation. Save for later measurement. Did solution change concentration? Solutes stay the same If water left or entered tissue –Water also entered or left solution –Solution becomes more dilute or.

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Auth with social network: Divide by 10 6 to convert to MPa. My presentations Profile Feedback Log out. A measure of how much material is packed into a unit volume of the material The fewer. Put two or preferably three potato cores in each solution water or sucrose. Cell Transport The movement of molecules can be either passive no energy or active needs energy depending upon the membrane structure and concentration.

Complete Tables 1 and 2. Boy, Oh Buoyancy Does it Float? Dispense 10 mL of water or a sucrose chqrdakov 0. Examine the cores as you weigh them.

Be sure not to include any fragments of the skin. OK Any substance that can charcakov shape or flow easily. Work quickly to minimize evaporation and keep the tissue wrapped in a moist towel. Does a gas differ from other fluids? In contrast to the Chardakov method which analyzes changes in solution density after incubation, this technique monitors tissue weight changes.

It is assumed that solute movement between tissue and solution is negligible. A “kink” may be observed in the graph below the x-axis. From the graph, determine the concentration of the sucrose solution in which there was no net weight gain i.


Cut them to the same length with a razor blade ca. Dispense 10 mL of water or sucrose 0. What would happen to The size of the tissue?

Feedback Privacy Policy Feedback. Weigh the cores to the nearest 0. Record your results Table 2 and repeat this chsrdakov for each of the sucrose solutions. Pour off the solutions into a set of empty, correspondingly labeled tubes. Do the cores show various degrees of turgor? Use the following equation to calculate the percent change in weight for each tissue by the following equation: What is the water potential of potato tissue?

Cytology Osmosis and Water relations. Work quickly to minimize evaporation and cnardakov the tissue wrapped in a moist towel.

Chardakov method

Water potential values determined by this method may be slightly more negative than those obtained kethod the Chardokov method. No change in density, concentration No net water movement Tissue and solution were at the same WP If we can find this solution concentration —We can find the tissue WP.

Hypertonic Concentration with higher solute concentration and less water concentration Hypotonic lower solute concentration and more water concentration.

Incubate the cores for at least 1. To use this website, you must agree to our Privacy Policyincluding methood policy. Immerse the pipette in the water that previously had tissue sections in it until the tip is approximately at the center of the tube.

Investigating osmosis: measuring the water potential of a potato cell

Record the temperature of the solutions Table 1 Using a Pasteur pipet, remove a small amount of water dyed with methylene blue to dye the sucrose solution, dip a dry probe into methylene blue powder and then mix.


This increases the tissue weight and may lead to small errors. The answer will be in Jm -3 which are equivalent to pressure Pa. Chradakov think you have liked this presentation. Use a cork borer to prepare at least 27 uniform tissue samples from the potato.

About project SlidePlayer Jethod of Service. What is the water potential calculated by this method? Alternately, for a more accurate measurement of changes in the solution density, a refractometer can be used. The water potential of the tissue is considered to be equal the osmotic potential of the incubating solution at which there is no change mehod tissue weight i.

Use a cork borer to prepare at least 27 uniform tissue samples from the potato. Change in weight of potato cores incubated in sucrose solutions.

One distinct advantage of this technique is that it provides a more accurate estimate of water potential. The Chardokov method provides a quick means to determine plant tissue water potentials. This occurs when the apoplast becomes infiltrated with water and solutes.

Registration Forgot your password? Measuring Water Potential by the Gravimetric Technique. An alternate method to determine his point requires performing a regression analysis of the best fit line of your data. Published by Sabrina Grant Modified over 2 years ago.

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