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Breve descripción acerca de los cromosomas politénicos de Drosophila melanogaster. View Notes – Laboratorio cromosomas politénicos (1).docx from BIO at Javeriana University. Laboratorio Identificacin de Cromosomas Politnicos de. Una herramienta importante para estos estudios es el etiquetado de los cromosomas politénicos con anticuerpos frente a la enzima, el factor de transcripción.

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Agradecemos a la Sra. Please recommend JoVE to your librarian. When you put them on the coverslip and then pick up the coverslip with the slide the chromosomes may float away to the edges of the cover slip if there is too much liquid present.

Cromosomas politénicos

You must be signed in to post a comment. Repita los pasos 4. Warm to disperse ploitenicos Triton X Fill out the form below to receive a free trial or learn more about access:. A subscription to J o VE is required to view this article. My thought is that they may be getting flushed out during my transfers.

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Deficiencia intelectual – catarata – cifosis – FEMEXER

If the problem continues, please let us know and we’ll try to help. Caliente para dispersar a la Triton X y su uso dentro de una hora.


If you have any question you can send me another e-mail. Is it the PFA?

The dissection forceps we use were mis-identified: Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes. The trick is to leave them in just long enough.

Los cromosomas politenicos como herramienta para el estudio de especies de la familia simulidae.

Examinar el portaobjetos una vez que el hielo se ha sublimado lejos de confirmar que el tejido adherido a la diapositiva y no quedarse con el cubreobjetos. If you leave the glands in too long they will fall apart.

Click here for the english version. Get cutting-edge science videos from J o VE sent straight to your inbox every month. If you are seeing few salivary gland chromosome sets you may be losing them when you squash.

Fill out the form below to receive a free trial or learn more about access: Las modificaciones de este procedimiento se describen en detalle en Johansen et al. An unexpected error occurred.

This may be a dumb question but when you put the tissue in Fixative 1 the Triton will lyse the membrane scorrect? You are right about the Triton. You will only be able to see the first 20 seconds. Please check your Internet connection and reload this page. Do you have any advice? Lugar pesos g en el cubreobjetos de las diapositivas. We recommend downloading the newest version of Flash here, but we support all versions 10 and above.


Please sign in or create an account. That is the volume I prepare each time, usually enough for two or three prep. A squash that is too rapid or hard may also cause the chromosomes to float away.

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Try placing them on the coverslip in a smaller drop of liquid and being careful about how you apply the pressure when you squash. Skip to content Biology. Coloque un segundo Kim-borrar en la parte superior y aplanar los cromosomas, colocando el pulgar sobre el lugar donde se coloca el cubreobjetos y presionar con firmeza, evitando cualquier movimiento horizontal del cubreobjetos que se cortelos cromosomas.